6Y1I

Human Eg5 motor domain mutant R234C

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2013-06-12
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97888
Spacegroup name	P 1 21 1
Unit cell lengths	87.428, 87.603, 95.065
Unit cell angles	90.00, 116.82, 90.00

Refinement procedure

Resolution	47.700 - 3.000
R-factor	0.2736
Rwork	0.272
R-free	0.30650
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ii6
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.700	3.107
High resolution limit [Å]	3.000	3.000
Rmerge	0.050	0.564
Rmeas	0.071	0.798
Rpim	0.050	0.564
Number of reflections	51247	5120
<I/σ(I)>	6.72	1.35
Completeness [%]	99.5	99.88
Redundancy	2	2
CC(1/2)	0.997	0.766

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	277.15	PEG3350, 0.15M Na tartrate, 0.1M MES pH 5.6