6Y16
CRYSTAL STRUCTURE OF TMARGBP DOMAIN 1 IN COMPLEX WITH THE GUANIDINIUM ION
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | Cu FINE FOCUS |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-06-06 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 |
| Unit cell lengths | 35.447, 45.812, 52.408 |
| Unit cell angles | 64.07, 70.33, 90.02 |
Refinement procedure
| Resolution | 23.630 - 1.700 |
| R-factor | 0.162 |
| Rwork | 0.161 |
| R-free | 0.18700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6gpc |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.623 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.760 |
| High resolution limit [Å] | 1.698 | 1.700 |
| Rmerge | 0.066 | 0.251 |
| Number of reflections | 25777 | 1392 |
| <I/σ(I)> | 20.2 | |
| Completeness [%] | 85.0 | 45 |
| Redundancy | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | THE BEST CRYSTALS OF D1 DOMAIN WERE OBTAINED USING A PROTEIN CONCENTRATION OF 8 MG/ML IN A SOLUTION CONTAINING 0.2 M NACL, 0.1M BIS-TRIS (PH 5.5), 25% (W/V) POLYETHYLENE GLYCOL (PEG) 3,350., VAPOR DIFFUSION, HANGING DROP, |
