6XZF

Nanobody in complex with eGFP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-04-27
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0332
Spacegroup name	P 31 2 1
Unit cell lengths	69.335, 69.335, 169.063
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.950 - 1.800
R-factor	0.1746
Rwork	0.173
R-free	0.20350
Structure solution method	SAD
RMSD bond length	0.011
RMSD bond angle	1.281
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	SHELXDE
Refinement software	PHENIX (1.16-3549)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.950	48.950	1.720
High resolution limit [Å]	1.690	9.090	1.690
Rmerge	0.066	0.037	2.140
Rmeas	0.068	0.038	2.295
Rpim	0.016	0.009	0.781
Total number of observations	989184	6624	14134
Number of reflections	52868	427	1831
<I/σ(I)>	22.4	69.8	0.9
Completeness [%]	98.3	99.4	68.7
Redundancy	18.7	15.5	7.7
CC(1/2)	1.000	0.999	0.113

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.1 M NaAcetate pH 4.6 8 % PEG 4000