6XZ5
RovC - regulator of virulence interconnected with the Csr system
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, DESY BEAMLINE P11 |
| Synchrotron site | PETRA III, DESY |
| Beamline | P11 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-09-05 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.033200 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 97.278, 97.278, 46.041 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 33.440 - 2.300 |
| R-factor | 0.231 |
| Rwork | 0.229 |
| R-free | 0.27390 |
| Structure solution method | SAD |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.372 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.29) |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 33.440 | 33.440 | 2.380 |
| High resolution limit [Å] | 2.300 | 8.910 | 2.300 |
| Rmerge | 0.083 | 0.020 | 0.566 |
| Rmeas | 0.096 | 0.023 | 0.657 |
| Rpim | 0.047 | 0.011 | 0.327 |
| Total number of observations | 796 | 4356 | |
| Number of reflections | 11309 | 210 | 1095 |
| <I/σ(I)> | 9.7 | 21.7 | 2.3 |
| Completeness [%] | 99.5 | 96.2 | 99.9 |
| Redundancy | 4 | 3.8 | 4 |
| CC(1/2) | 0.996 | 0.999 | 0.694 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 293.15 | 0.2 M KCl, 0.01 M MgSO4 , 0.01 M MES pH 5.6, 10% PEG400 |
