6XZ3
Crystal structure of TLNRD1 4-helix bundle
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-11-14 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97855 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 114.595, 114.595, 59.404 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 57.360 - 2.190 |
| R-factor | 0.2152 |
| Rwork | 0.213 |
| R-free | 0.25330 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2x0c |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.639 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.360 | 2.310 |
| High resolution limit [Å] | 2.188 | 2.188 |
| Rmerge | 0.130 | 1.034 |
| Rmeas | 0.135 | 1.074 |
| Rpim | 0.037 | 0.288 |
| Number of reflections | 10508 | 1491 |
| <I/σ(I)> | 12.5 | 2.6 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 13.4 | 13.6 |
| CC(1/2) | 0.996 | 0.938 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 294 | 0.3 M ammonium citrate dibasic, 25% PEG 3350 |
