6XZ3

Crystal structure of TLNRD1 4-helix bundle

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-11-14
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97855
Spacegroup name	I 41 2 2
Unit cell lengths	114.595, 114.595, 59.404
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	57.360 - 2.190
R-factor	0.2152
Rwork	0.213
R-free	0.25330
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2x0c
RMSD bond length	0.009
RMSD bond angle	1.639
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	57.360	2.310
High resolution limit [Å]	2.188	2.188
Rmerge	0.130	1.034
Rmeas	0.135	1.074
Rpim	0.037	0.288
Number of reflections	10508	1491
<I/σ(I)>	12.5	2.6
Completeness [%]	100.0	99.9
Redundancy	13.4	13.6
CC(1/2)	0.996	0.938

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		294	0.3 M ammonium citrate dibasic, 25% PEG 3350