6XYX

Crystal structure of the BCL6 BTB domain in complex with the NCoR1 BBD corepressor peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-12-15
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	34.660, 64.520, 137.750
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	21.630 - 1.440
R-factor	0.1686
Rwork	0.167
R-free	0.19780
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1r28
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	68.880	1.520
High resolution limit [Å]	1.440	1.440
Rmerge	0.059
Rpim	0.034	0.894
Number of reflections	56791	8133
<I/σ(I)>	9.1	1.4
Completeness [%]	100.0
Redundancy	3.8
CC(1/2)	0.998	0.606

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	298	0.2 M Sodium acetate trihydrate 0.1 M Bis-Tris propane 7.5 20 % w/v PEG 3350