Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-01-19 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 94.620, 94.620, 159.010 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.180 - 2.460 |
| R-factor | 0.2326 |
| Rwork | 0.229 |
| R-free | 0.29620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qo9 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.044 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.180 | 2.543 |
| High resolution limit [Å] | 2.455 | 2.455 |
| Rmerge | 0.049 | 0.335 |
| Rmeas | 0.052 | 0.387 |
| Rpim | 0.018 | 0.185 |
| Number of reflections | 26803 | 2542 |
| <I/σ(I)> | 20.7 | 3.3 |
| Completeness [%] | 98.5 | |
| Redundancy | 6 | |
| CC(1/2) | 0.999 | 0.939 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 292 | 13% MPEG2000, 0.1 M ammonium sulfate, 0.03 M leucine, 0.1 M acetate, pH 4.6 |
