6XYR

Structure of the T4Lnano fusion protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-03-03
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.0
Spacegroup name	P 21 21 2
Unit cell lengths	86.380, 104.260, 63.950
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.100 - 2.079
R-factor	0.1928
Rwork	0.191
R-free	0.23570
Structure solution method	SAD
RMSD bond length	0.011
RMSD bond angle	1.038
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.100	2.154
High resolution limit [Å]	2.079	2.079
Rmerge	0.128	2.043
Rpim	0.037	0.582
Number of reflections	35404	3434
<I/σ(I)>	14.53	1.31
Completeness [%]	99.8
Redundancy	13.1
CC(1/2)	0.999	0.600

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	8% PEG 8000, 200 mM LiCl2, 100 mM Tris pH 8.0, 15% Glycerol