6XYD
Crystal structure of Q4D6Q6, a conserved kinetoplastid-specific protein from Trypanosoma cruzi
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-08-26 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.918410 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 66.213, 44.771, 68.654 |
| Unit cell angles | 90.00, 93.40, 90.00 |
Refinement procedure
| Resolution | 37.070 - 1.810 |
| R-factor | 0.185 |
| Rwork | 0.183 |
| R-free | 0.21600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6xyb |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.850 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.070 | 1.920 |
| High resolution limit [Å] | 1.810 | 1.810 |
| Rmeas | 0.085 | 0.881 |
| Number of reflections | 36826 | 5892 |
| <I/σ(I)> | 12.9 | 1.82 |
| Completeness [%] | 99.5 | 99.4 |
| Redundancy | 3.3 | 3.3 |
| CC(1/2) | 0.998 | 0.684 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 20% PEG 3350, 0.2 M sodium acetate |
