6XY4

Structural insight into sheep-pox virus mediated inhibition of apoptosis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-07-18
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9537
Spacegroup name	C 2 2 21
Unit cell lengths	43.280, 70.660, 115.080
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.066 - 2.046
R-factor	0.220603579671
Rwork	0.220
R-free	0.23381
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2bjy
RMSD bond length	0.002
RMSD bond angle	0.398
Data reduction software	XDS (20180427)
Data scaling software	Aimless (7.0)
Phasing software	PHASER (1.11.1_2575-000)
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.070	8.920
High resolution limit [Å]	2.046	2.050
Rmerge	0.066
Number of reflections	11236	782
<I/σ(I)>	8.7
Completeness [%]	98.2
Redundancy	5
CC(1/2)	0.990	0.573

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	0.2 M Potassium sodium tartrate tetrahydrate, 0.1 M Bis-Tris propane pH 6.5, 20% w/vPEG 3350