6XXK

Crystal Structure of Human Deoxyhypusine Synthase in complex with spermidine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-02-08
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9184
Spacegroup name	P 32 2 1
Unit cell lengths	104.980, 104.980, 160.898
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.194 - 1.650
R-factor	0.1554
Rwork	0.155
R-free	0.16220
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1dhs
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER (v.1.17.1)
Refinement software	PHENIX (v.1.17.1)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.194	46.194	1.750
High resolution limit [Å]	1.649	4.920	1.650
Rmerge	0.110	0.044	2.352
Rmeas	0.116	0.046	2.465
Number of reflections	123691	4959	19734
<I/σ(I)>	12.8	49.74	0.94
Completeness [%]	99.9	99.8	99.2
Redundancy	11.092	10.264	11.164
CC(1/2)	0.999	0.999	0.672

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.025-0.125 mM carboxylic acid mix, 30-60% precipitant mix, 100 mM Tris-Bicine pH = 8.5