6XXH

Crystal Structure of Human Deoxyhypusine Synthase in apo form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-09
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9184
Spacegroup name	P 32 2 1
Unit cell lengths	104.969, 104.969, 161.026
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.220 - 1.520
R-factor	0.1545
Rwork	0.154
R-free	0.17620
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1dhs
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASES (1.17.1_3660)
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.220	46.220	1.610
High resolution limit [Å]	1.520	4.540	1.520
Rmerge	0.106	0.037	3.046
Rmeas	0.111	0.039	3.207
Total number of observations	1592643
Number of reflections	157775	6277	25204
<I/σ(I)>	13.09	57.52	0.76
Completeness [%]	99.9	99.8	99.7
Redundancy	10.094	9.72	10.202
CC(1/2)	0.999	0.999	0.434

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.025-0.125 mM carboxylic acid mix, 30-60% precipitant mix (MPD, PEG 1000, PEG 3350), 100 mM Tris-Bicine pH = 8.5