6XXF

1.7 Angstrom crystal structure of Ca/CaM:RyR2 peptide complex

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-04-04
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97857
Spacegroup name	P 21 21 21
Unit cell lengths	39.916, 41.710, 85.986
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.556 - 1.700
Rwork	0.180
R-free	0.20520
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2bcx
RMSD bond length	0.013
RMSD bond angle	1.805
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.710	1.740
High resolution limit [Å]	1.700	1.700
Rmerge	0.092	0.544
Rmeas	0.111	0.664
Rpim	0.061	0.375
Number of reflections	16464	1201
<I/σ(I)>	12.5	6.2
Completeness [%]	100.0	99.9
Redundancy	5.9	5.7
CC(1/2)	0.991	0.870

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1M sodium acetate pH4.5, 0.2M ammonium acetate, 10% PEG4000