6XX8

Arabidopsis thaliana Casein Kinase 2 (CK2) alpha-1 crystal form II

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-04-01
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	0.9801
Spacegroup name	P 1 21 1
Unit cell lengths	47.850, 81.170, 83.460
Unit cell angles	90.00, 105.17, 90.00

Refinement procedure

Resolution	46.180 - 1.800
R-factor	0.209
Rwork	0.207
R-free	0.24580
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3pzh
RMSD bond length	0.006
RMSD bond angle	0.959
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER (1.9_1692)
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.200	1.890
High resolution limit [Å]	1.780	1.780
Number of reflections	57878	8433
<I/σ(I)>	12
Completeness [%]	99.8
Redundancy	7.1
CC(1/2)	0.998	0.175

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2M sodium sulfate 0.1M Bis-Tris propane pH 6.5 20% PEG 3350