6XX6

Arabidopsis thaliana Casein Kinase 2 (CK2) alpha-1 crystal form I

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-04-01
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	0.9801
Spacegroup name	P 41 21 2
Unit cell lengths	61.980, 61.980, 227.730
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.010 - 1.849
R-factor	0.174441353737
Rwork	0.173
R-free	0.19906
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3pzh
RMSD bond length	0.006
RMSD bond angle	1.065
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER (1.11.1_2575)
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.960
High resolution limit [Å]	1.849	1.850
Number of reflections	38648	5815
<I/σ(I)>	13.4
Completeness [%]	98.7
Redundancy	8.4
CC(1/2)	0.998	0.704

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	0.2M sodium sulfate 0.1M Bis-Tris propane pH 6.5 20% PEG 3350