Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-08-10 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.84920 |
| Spacegroup name | P 63 |
| Unit cell lengths | 106.320, 106.320, 58.670 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.390 - 1.170 |
| Rwork | 0.119 |
| R-free | 0.14500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4yjj |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.747 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.390 | 1.200 |
| High resolution limit [Å] | 1.170 | 1.170 |
| Number of reflections | 127070 | 127070 |
| <I/σ(I)> | 21.3 | |
| Completeness [%] | 100.0 | |
| Redundancy | 14 | |
| CC(1/2) | 1.000 | 0.510 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 294 | MPD, PEG1000, PEG3350, Diethylene glycol, Triethylene glycol, Tetraethylene glycol, Pentaethylene glycol |
