6XUT

Crystallographic structure of oligosaccharide dehydrogenase from Pycnoporus cinnabarinus, ligand-free form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-28
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	48.874, 61.592, 195.091
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	97.550 - 1.600
R-factor	0.16626
Rwork	0.165
R-free	0.19648
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ynt
RMSD bond length	0.013
RMSD bond angle	1.713
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	97.740	1.570
High resolution limit [Å]	1.430	1.430
Number of reflections	103248	25836
<I/σ(I)>	14.5	1.72
Completeness [%]	99.9
Redundancy	12.7
CC(1/2)	1.000	0.790

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		294	2 M ammonium sulfate