6XU9
Human myelin protein P2 mutant K3N
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X12 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X12 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-03-24 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.91 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 64.520, 64.520, 101.210 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.700 |
| R-factor | 0.2143 |
| Rwork | 0.212 |
| R-free | 0.24980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wut |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.511 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (dev-2247) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.770 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmeas | 0.181 | 0.838 |
| Number of reflections | 6311 | 454 |
| <I/σ(I)> | 14.5 | 3.1 |
| Completeness [%] | 99.5 | 100 |
| Redundancy | 10.7 | 11.2 |
| CC(1/2) | 0.997 | 0.950 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 277 | 35% PEG 6000, 0.1 M citrate pH 5.0 |
