6XT7

Tel25 Hybrid Four-quartet G-quadruplex with K+

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	196
Detector technology	CCD
Collection date	2020-02-13
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9791
Spacegroup name	P 1 21 1
Unit cell lengths	29.998, 92.916, 50.081
Unit cell angles	90.00, 99.62, 90.00

Refinement procedure

Resolution	49.380 - 1.560
R-factor	0.1552
Rwork	0.154
R-free	0.18250
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1jrn
RMSD bond length	0.012
RMSD bond angle	1.360
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.18_3845)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	93.060	93.060	1.650
High resolution limit [Å]	1.560	4.940	1.560
Rmerge	0.071	0.059	0.787
Rmeas	0.082	0.066	1.000
Rpim	0.030	0.025	0.385
Number of reflections	37569	1249	5351
<I/σ(I)>	15.4	44.3	2
Completeness [%]	98.0	99.7	96.1
Redundancy	6.6	6.5	6.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		296	39% 2-methyl-2,4-pentanediol, 0.165 M potassium chloride, 0.02 M magnesium chloride, 0.04 M sodium cacodylate pH 6.5, 0.012 M spermine tetrahydrochloride