6XSX
The structure of the catalytic module of the metalloprotease ZmpA from Clostridium perfringens
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-05-10 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97530 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.168, 54.553, 90.984 |
| Unit cell angles | 90.00, 93.20, 90.00 |
Refinement procedure
| Resolution | 30.780 - 2.100 |
| R-factor | 0.191 |
| Rwork | 0.189 |
| R-free | 0.22770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5kdn |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.105 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 2.140 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.212 | 0.960 |
| Rpim | 0.084 | 0.386 |
| Number of reflections | 34596 | 1700 |
| <I/σ(I)> | 9.7 | 2.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.4 | 7.2 |
| CC(1/2) | 0.963 | 0.734 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 0.1 M MES:NaOH and 12% PEG 20000 |
