6XSX

The structure of the catalytic module of the metalloprotease ZmpA from Clostridium perfringens

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-05-10
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97530
Spacegroup name	P 1 21 1
Unit cell lengths	60.168, 54.553, 90.984
Unit cell angles	90.00, 93.20, 90.00

Refinement procedure

Resolution	30.780 - 2.100
R-factor	0.191
Rwork	0.189
R-free	0.22770
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5kdn
RMSD bond length	0.004
RMSD bond angle	1.105
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.000	2.140
High resolution limit [Å]	2.100	2.100
Rmerge	0.212	0.960
Rpim	0.084	0.386
Number of reflections	34596	1700
<I/σ(I)>	9.7	2.3
Completeness [%]	100.0	100
Redundancy	7.4	7.2
CC(1/2)	0.963	0.734

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	291	0.1 M MES:NaOH and 12% PEG 20000