6XSV
X-ray structure of a tetragonal crystal form of alpha amylase from Aspergillus oryzae (Tala-Amylase) at 1.65 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 173 |
| Detector technology | PIXEL |
| Collection date | 2019-02-22 |
| Detector | DECTRIS PILATUS 300K |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 63.126, 63.126, 265.812 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 66.450 - 1.650 |
| R-factor | 0.1762 |
| Rwork | 0.175 |
| R-free | 0.20480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2taa |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | SIMBAD |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 66.450 | 1.680 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.135 | |
| Rmeas | 0.141 | |
| Rpim | 0.027 | |
| Number of reflections | 66065 | 3098 |
| <I/σ(I)> | 16.7 | 1.1 |
| Completeness [%] | 99.8 | 96.8 |
| Redundancy | 124.3 | 41 |
| CC(1/2) | 0.999 | 0.190 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | Crystals grown by sitting drop vapor diffusion in Cryschem plates using 0.6 ml reservoirs of 20% PEG 3350 in 0.1 M MES buffer at pH 6.5. Drops composed of equal amounts of the protein at 30 mg/ml in water with the reservoir solution. Crystals grew after about 0ne to two weeks at 298 K |
