6XSU

GH5-4 broad specificity endoglucanase from Ruminococcus flavefaciens

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2015-08-15
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97856
Spacegroup name	P 1 21 1
Unit cell lengths	50.232, 78.303, 82.415
Unit cell angles	90.00, 92.08, 90.00

Refinement procedure

Resolution	56.750 - 1.410
R-factor	0.1495
Rwork	0.149
R-free	0.17050
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1edg
RMSD bond length	0.007
RMSD bond angle	0.904
Data reduction software	DIALS
Data scaling software	DIALS
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	56.750	1.460
High resolution limit [Å]	1.410	1.410
Rmerge	0.123	0.405
Rmeas	0.141	0.499
Rpim	0.067	0.284
Number of reflections	118191	9774
<I/σ(I)>	5.38	0.93
Completeness [%]	96.2	79.24
Redundancy	4	2.4
CC(1/2)	0.991	0.729

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	1 microliter of protein at XXXX was combined with 1 microliter of reservoir solution consisting of 31%w/v PEG3350, 0.2 M NaCl, 0.1M bistris pH 5.5. Sample was cryoproected by brief exposure to ethanol vapor.