6XSO
GH5-4 broad specificity endoglucanase from an uncultured bacterium
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-03-05 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.774901 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 55.640, 91.890, 170.850 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.520 - 1.500 |
R-factor | 0.148 |
Rwork | 0.148 |
R-free | 0.16990 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3vdh |
RMSD bond length | 0.007 |
RMSD bond angle | 0.967 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASES |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 36.520 | 1.554 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.074 | 1.390 |
Rmeas | 0.077 | 1.444 |
Rpim | 0.021 | 0.386 |
Number of reflections | 140750 | 13918 |
<I/σ(I)> | 19.45 | 1.66 |
Completeness [%] | 100.0 | 99.94 |
Redundancy | 13.6 | 13.9 |
CC(1/2) | 1.000 | 0.746 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | Protein 37 mg/mL in 10 mM MOPS buffer at pH 7, 50 mM NaCl was mixed with an equal volume of reservoir solution with 0.15M DL Malic acid, 20% PEG 3350. Sample was cryoprotected with reservoir supplemented to 35% PEG3350 |