6XSG
Crystal structure of Staphylococcal nuclease variant Delta+PHS V66T at cryogenic temperature
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | OTHER |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2013-01-11 |
| Detector | APEX II CCD |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 30.950, 60.281, 37.518 |
| Unit cell angles | 90.00, 93.03, 90.00 |
Refinement procedure
| Resolution | 31.840 - 2.000 |
| R-factor | 0.2006 |
| Rwork | 0.198 |
| R-free | 0.24740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3bdc |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.607 |
| Data reduction software | APEX 2 |
| Data scaling software | SAINT (2013/1 for Windows) |
| Phasing software | PHASER (2.8.2) |
| Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.060 | 0.449 |
| Number of reflections | 9321 | 383 |
| <I/σ(I)> | 25.21 | 3.12 |
| Completeness [%] | 99.3 | 99.5 |
| Redundancy | 12.62 | 6.33 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 277 | 32% MPD, 25 mM potassium phosphate, calcium chloride, THP |
