6XSE
Crystal structure of Staphylococcal nuclease variant Delta+PHS F34Y at cryogenic temperature
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | OTHER |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2018-03-16 |
| Detector | AGILENT ATLAS CCD |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 30.958, 61.309, 38.051 |
| Unit cell angles | 90.00, 92.98, 90.00 |
Refinement procedure
| Resolution | 24.630 - 2.100 |
| R-factor | 0.2331 |
| Rwork | 0.231 |
| R-free | 0.27910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3bdc |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.540 |
| Data reduction software | CrysalisPro |
| Data scaling software | CrysalisPro |
| Phasing software | PHASER (2.8.2) |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.021 | 0.143 |
| Number of reflections | 8368 | 844 |
| <I/σ(I)> | 41.1 | 5.77 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 10.6 | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 277 | 26% MPD, 25 mM potassium phosphate, calcium chloride, THP |
