6XSC
Crystal structure of Staphylococcal nuclease variant Delta+PHS V23T at cryogenic temperature
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SEALED TUBE |
Source details | Agilent SuperNova |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2018-03-16 |
Detector | AGILENT ATLAS CCD |
Wavelength(s) | 1.54 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 31.030, 60.530, 38.097 |
Unit cell angles | 90.00, 93.20, 90.00 |
Refinement procedure
Resolution | 38.040 - 2.000 |
R-factor | 0.2136 |
Rwork | 0.211 |
R-free | 0.25860 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3bdc |
RMSD bond length | 0.013 |
RMSD bond angle | 1.686 |
Data reduction software | APEX 2 |
Data scaling software | CrysalisPro |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.040 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.064 | 0.276 |
Number of reflections | 9461 | 961 |
<I/σ(I)> | 37.26 | 4.79 |
Completeness [%] | 99.9 | 100 |
Redundancy | 10.1 | 6.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 20% MPD, 25 mM potassium phosphate, calcium chloride |