6XRW

Chromosomal ParDE TA system from P. aeruginosa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-07-25
Detector	DECTRIS PILATUS 200K
Wavelength(s)	1.54
Spacegroup name	C 2 2 21
Unit cell lengths	98.401, 121.323, 58.584
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.860 - 1.770
R-factor	0.1799
Rwork	0.177
R-free	0.21920
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5cze
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.830
High resolution limit [Å]	1.770	4.880	1.800
Rmerge	0.048	0.028	0.276
Rmeas	0.054	0.031	0.352
Rpim	0.022	0.014	0.216
Number of reflections	33497	1774	1574
<I/σ(I)>	12.8
Completeness [%]	99.6	99.7	95.3
Redundancy	5.1	5.3	2.2
CC(1/2)		0.999	0.900

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	1:1 mixture of precipitant 7.5% MPD, 20% PEG 6000, 25 mM (NH4)2SO4, 100 mM Tris pH 7.0 with protein solution 10.5 mg/mL ParDE in 25 mM MES pH 6.0, 150 mM NaCl, 2.5% glycerol