6XRK

GH5-4 broad specificity endoglucanase from an uncultured bovine rumen ciliate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-03-05
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.774901
Spacegroup name	P 2 21 21
Unit cell lengths	72.320, 84.990, 121.320
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.640 - 1.419
R-factor	0.145
Rwork	0.145
R-free	0.16700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2jep
RMSD bond length	0.007
RMSD bond angle	0.954
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.640	1.470
High resolution limit [Å]	1.419	1.420
Rmerge	0.077	1.415
Number of reflections	141292	13953
<I/σ(I)>	18.36	1.28
Completeness [%]	100.0	99.9
Redundancy	13.7	14.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	200 nL 37 mg/mL protein, 50 mM sodium chloride, 10 mM MOPS, pH 7 + 200 nL reservoir solution (200 mM ammonium acetate, 100 mM Bis-Tris methane, pH 6.5, 25% PEG3350), MRC SD2 plate, cryoprotectant: reservoir solution + 35% PEG3350