6XRF
EagT6 Tse6 NT complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 95 |
| Detector technology | IMAGE PLATE |
| Collection date | 2019-09-12 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 32 |
| Unit cell lengths | 68.951, 68.951, 173.054 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.840 - 2.560 |
| R-factor | 0.2316 |
| Rwork | 0.230 |
| R-free | 0.26820 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.724 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.840 | 2.660 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Rmeas | 0.183 | 1.980 |
| Number of reflections | 29267 | 2832 |
| <I/σ(I)> | 6.89 | |
| Completeness [%] | 99.2 | 96.4 |
| Redundancy | 4.9 | 4.26 |
| CC(1/2) | 0.996 | 0.502 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 278 | 20 mg/mL EagT6:Tse6(NT) with a 1:1 mixture of 0.2M Magnesium chloride, 0.1M Bis-TRIS pH 5.5, and 25% (w/v) PEG 3350 |
