6XQ9

Receptor for Advanced Glycation End Products VC1 domain in complex with Fragments 1 & 13

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2018-07-24
Detector	RAYONIX MX-300
Wavelength(s)	0.97857
Spacegroup name	P 62
Unit cell lengths	102.112, 102.112, 102.179
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	34.060 - 2.300
R-factor	0.2159
Rwork	0.215
R-free	0.23880
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4lp4
RMSD bond length	0.009
RMSD bond angle	1.327
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.380
High resolution limit [Å]	2.300	2.300
Rmeas	0.063	0.166
Rpim	0.019	0.050
Number of reflections	52826	5258
<I/σ(I)>	29.9	18.38
Completeness [%]	99.7	98.43
Redundancy	5.7	5.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	2.5 M NaOAc (pH 7.5)