6XQ7

Receptor for Advanced Glycation End Products VC1 domain in complex with Fragment 5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 12.3.1
Synchrotron site	ALS
Beamline	12.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2018-08-15
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9795
Spacegroup name	P 62
Unit cell lengths	102.905, 102.905, 101.769
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.920 - 1.800
R-factor	0.2002
Rwork	0.199
R-free	0.22200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4lp4
RMSD bond length	0.018
RMSD bond angle	1.622
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.860
High resolution limit [Å]	1.800	1.800
Rmeas	0.070	0.310
Rpim	0.025	0.110
Number of reflections	111660	11201
<I/σ(I)>	17.1	7.83
Completeness [%]	99.8	99.63
Redundancy	3.9	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	2.5 M NaOAc (pH 7.5)