6XQ5

Receptor for Advanced Glycation End Products VC1 domain in complex with Fragment 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2016-02-15
Detector	RAYONIX MX-300
Wavelength(s)	0.97872
Spacegroup name	P 62
Unit cell lengths	101.735, 101.735, 101.812
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.500 - 1.800
R-factor	0.1743
Rwork	0.173
R-free	0.19630
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4lp4
RMSD bond length	0.016
RMSD bond angle	1.359
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.860
High resolution limit [Å]	1.800	1.800
Rmeas	0.067	0.356
Rpim	0.024	0.128
Number of reflections	55196	5530
<I/σ(I)>	15.6	7.96
Completeness [%]	97.2
Redundancy	4.4	4.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	2.5 M NaOAc (pH 7.5)