6XPK

CutR Screw, form 1 with 41.9 angstrom pitch

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-11
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9791
Spacegroup name	P 61
Unit cell lengths	61.830, 61.830, 41.930
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	53.546 - 2.800
Rwork	0.231
R-free	0.26690
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6xph
RMSD bond length	0.002
RMSD bond angle	1.234
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER (2.8.3)
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	53.546	53.546	2.870
High resolution limit [Å]	2.800	12.510	2.800
Rmerge	0.113	0.042	0.954
Rmeas	0.119	0.044	1.009
Number of reflections	2305	28	161
<I/σ(I)>	15.46	40.1	2.22
Completeness [%]	99.4	100	95.8
Redundancy	9.512	8.571	9.379
CC(1/2)	0.999	1.000	0.722

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.2	298	10% (w/v) PEG 3000, 200mM sodium chloride and 100 mM sodium phosphate dibasic/Citric acid pH 4.2