6XPJ

CutR flat hexamer, form 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-12-12
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.979300
Spacegroup name	P 42 21 2
Unit cell lengths	79.290, 79.290, 100.790
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	62.318 - 1.500
Rwork	0.161
R-free	0.18410
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6xpk
RMSD bond length	0.010
RMSD bond angle	1.600
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER (2.8.3)
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	62.320	62.320	1.540
High resolution limit [Å]	1.500	6.710	1.500
Rmerge	0.059	0.044	0.855
Rmeas	0.061	0.046	0.891
Number of reflections	52024	688	3754
<I/σ(I)>	23.7	62.09	3.08
Completeness [%]	99.9	99.3	99.3
Redundancy	12.954	11.557	12.655
CC(1/2)	0.999	0.999	0.860

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	10.5	298	2M ammonium sulfate, 0.2M lithium sulfate and 0.1M CAPS/Sodium hydroxide pH 10.5