6XO5
Self-assembly of a 3D DNA crystal lattice (4x6 junction version) containing the J8 immobile Holliday junction
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-11-15 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1 |
| Spacegroup name | P 32 |
| Unit cell lengths | 68.304, 68.304, 54.283 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.152 - 3.157 |
| R-factor | 0.2602 |
| Rwork | 0.258 |
| R-free | 0.29510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6xna |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.584 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.200 |
| High resolution limit [Å] | 3.150 | 8.540 | 3.150 |
| Rmerge | 0.082 | 0.050 | 0.462 |
| Rmeas | 0.092 | 0.055 | 0.545 |
| Rpim | 0.040 | 0.023 | 0.279 |
| Total number of observations | 17812 | ||
| Number of reflections | 3906 | 244 | 112 |
| <I/σ(I)> | 7.5 | ||
| Completeness [%] | 80.0 | 98.8 | 44.3 |
| Redundancy | 4.6 | 5.8 | 2.4 |
| CC(1/2) | 0.999 | 0.870 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.5 mL of 0.05 M Cacodylate pH 6.0 with 200 mM Ca(CH3COO)2 and 2.5 M NaCl was added to the reservoir with 2 uL added to the drop containing 4 uL of DNA stock |
