6XNV

CRYSTAL STRUCTURE OF LISTERIA MONOCYTOGENES CBPB PROTEIN (LMO1009) IN COMPLEX WITH C-DI-AMP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-08-17
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97920
Spacegroup name	P 43 21 2
Unit cell lengths	61.934, 61.934, 171.509
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.930 - 2.400
R-factor	0.2172
Rwork	0.211
R-free	0.27580
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3lqn
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	25.000	25.000	2.490
High resolution limit [Å]	2.400	5.160	2.400
Rmerge	0.077	0.056	0.474
Rmeas	0.087	0.064	0.536
Rpim	0.039	0.029	0.245
Total number of observations	64523
Number of reflections	13591	1501	1250
<I/σ(I)>	9
Completeness [%]	98.2	97.5	92.9
Redundancy	4.7	4.5	4.2
CC(1/2)		0.994	0.827

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	8	298	200 mM CaCl2, 100 mM imidazole, pH 8.0, 14% PEG 8,000