6XML
Human aldolase A I98C
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-07-12 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9795 |
Spacegroup name | P 64 2 2 |
Unit cell lengths | 162.571, 162.571, 167.797 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 40.200 - 1.880 |
R-factor | 0.247 |
Rwork | 0.245 |
R-free | 0.28560 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ald |
RMSD bond length | 0.024 |
RMSD bond angle | 1.862 |
Refinement software | REFMAC (1.16_3549) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 107.850 | 2.080 |
High resolution limit [Å] | 1.880 | 1.880 |
Rpim | 0.027 | 0.347 |
Number of reflections | 65292 | 3266 |
<I/σ(I)> | 14.4 | |
Completeness [%] | 94.6 | |
Redundancy | 9.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 277 | 10% (w/v) PEG 8000, 21% (v/v) glycerol in 0.04 M potassium phosphate, pH 6.0 |