6XMB
Structure of an anophensin from Anopheles stephensi
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-10-20 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.000 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 67.280, 82.360, 71.730 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.710 - 2.310 |
R-factor | 0.2305 |
Rwork | 0.229 |
R-free | 0.25870 |
Structure solution method | SAD |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 35.710 | 35.710 | 2.370 |
High resolution limit [Å] | 2.310 | 10.330 | 2.310 |
Rmerge | 0.059 | 0.056 | 0.577 |
Rmeas | 0.063 | 0.062 | 0.618 |
Total number of observations | 142373 | ||
Number of reflections | 18055 | 244 | 1309 |
<I/σ(I)> | 19.44 | 36.83 | 4.12 |
Completeness [%] | 100.0 | 98.4 | 100 |
Redundancy | 7.886 | 5.963 | 7.868 |
CC(1/2) | 0.997 | 0.995 | 0.951 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 7% PEG10000, 0.1 M Tris-HCl |