6XMB

Structure of an anophensin from Anopheles stephensi

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-10-20
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.000
Spacegroup name	P 21 21 2
Unit cell lengths	67.280, 82.360, 71.730
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.710 - 2.310
R-factor	0.2305
Rwork	0.229
R-free	0.25870
Structure solution method	SAD
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.18.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	35.710	35.710	2.370
High resolution limit [Å]	2.310	10.330	2.310
Rmerge	0.059	0.056	0.577
Rmeas	0.063	0.062	0.618
Total number of observations	142373
Number of reflections	18055	244	1309
<I/σ(I)>	19.44	36.83	4.12
Completeness [%]	100.0	98.4	100
Redundancy	7.886	5.963	7.868
CC(1/2)	0.997	0.995	0.951

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	298	7% PEG10000, 0.1 M Tris-HCl