6XMA

Crystal structure of iron-bound LSD4 from Sphingobium sp. strain SYK-6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	CCD
Collection date	2019-06-10
Detector	RIGAKU SATURN 944+
Wavelength(s)	1.5418
Spacegroup name	I 2 2 2
Unit cell lengths	86.030, 112.527, 115.384
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.788 - 1.450
R-factor	0.1503
Rwork	0.149
R-free	0.17070
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6ojr
Data reduction software	DENZO
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.14-3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.480
High resolution limit [Å]	1.450	3.940	1.450
Rmerge	0.110	0.072	0.558
Rmeas	0.113	0.073	0.633
Rpim	0.024	0.012	0.291
Number of reflections	190316	5203	4752
<I/σ(I)>	12.7
Completeness [%]	99.9	99.8	97.3
Redundancy	16.1	33.9	4.2
CC(1/2)		0.999	0.829

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293	0.4 M magnesium acetate and 28% PEG3350