6XKS

Crystal structure of domain A from the periplasmic Lysine-, Arginine-, Ornithine-binding protein (LAO) of Salmonella typhimurium

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-04-26
Detector	DECTRIS PILATUS 200K
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	46.597, 87.785, 109.154
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.860 - 2.400
R-factor	0.2361
Rwork	0.230
R-free	0.28800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2lao
RMSD bond length	0.004
RMSD bond angle	0.678
Data reduction software	CrystalClear
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.440
High resolution limit [Å]	2.400	2.400
Rmerge	0.160	0.826
Number of reflections	32910	1691
<I/σ(I)>	16.6	2
Completeness [%]	99.3	98.6
Redundancy	6.7	4.7
CC(1/2)	0.991	0.694

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	291.15	0.2 M Sodium acetate trihydrate, 0.1 M Sodium cacodylate trihydrate pH 6.5, 30% w/v Polyethylene glycol 8,000