6XKA

TPX2 residues 7-20 fused to Aurora A residues 116-389 dephosphorylated, and CoAlated on C290

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-27
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 41 21 2
Unit cell lengths	86.310, 86.310, 76.834
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.790 - 2.650
R-factor	0.2258
Rwork	0.220
R-free	0.27890
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6vpg
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.740
High resolution limit [Å]	2.650	5.710	2.650
Rmerge	0.064	0.051	0.807
Rmeas	0.070	0.056	0.890
Rpim	0.028	0.022	0.368
Total number of observations	53014
Number of reflections	8813	964	848
<I/σ(I)>	6.7
Completeness [%]	99.2	97.1	99.2
Redundancy	6	5.7	5.5
CC(1/2)		0.988	0.632

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		298	0.2 M sodium citrate pH 4, 0.1 M sodium citrate pH 5.5, 18 mM sodium malonate pH 6, 4 mM MgCl2