6XJO

Human atlastin-3 (residues 1-334) bound to GDP-Mg2+ exhibits an ordered amino terminus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-11-22
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.977
Spacegroup name	P 21 21 21
Unit cell lengths	44.480, 88.040, 168.820
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.020 - 2.100
R-factor	0.1812
Rwork	0.178
R-free	0.23080
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5vgr
RMSD bond length	0.008
RMSD bond angle	0.911
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.150
High resolution limit [Å]	2.100	2.100
Rmeas	0.105	1.517
Number of reflections	39632	2864
<I/σ(I)>	14.7
Completeness [%]	99.9
Redundancy	8
CC(1/2)	0.990	0.665

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293.15	187.5 mM Ammonium acetate, 21.5% PEG3350, 100 mM Bis-Tris pH 6.5