6XJL

Structure of the SM protein Vps45

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-11-06
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9782
Spacegroup name	P 21 21 21
Unit cell lengths	68.489, 93.956, 102.615
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.650 - 2.000
R-factor	0.1814
Rwork	0.179
R-free	0.22100
Structure solution method	SAD
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELXDE
Refinement software	PHENIX (1.17_3644)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	35.000	35.000	2.030
High resolution limit [Å]	2.000	5.420	2.000
Rmerge	0.082	0.035	0.708
Rmeas	0.092	0.040	0.807
Rpim	0.042	0.019	0.383
Total number of observations	209783
Number of reflections	45356	2474	2211
<I/σ(I)>	6.1
Completeness [%]	99.6	99.6	98.9
Redundancy	4.6	4.3	4.2
CC(1/2)		0.998	0.743

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	0.2 M potassium bromide, 0.2 M potassium thiocyanate, 0.1 M sodium acetate pH 6.0, 3% (w/v) polyglycolide, 5% (w/v) polyethylene glycol 3350