6XJ6

Crystal structure of the helical cell shape determining protein Pgp2 from Campylobacter jejuni

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-02-14
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	47.451, 71.942, 91.691
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.971 - 1.497
R-factor	0.1594
Rwork	0.158
R-free	0.19150
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4xzz
RMSD bond length	0.004
RMSD bond angle	0.709
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (v1.12-2829)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.530
High resolution limit [Å]	1.497	4.070	1.500
Rmerge	0.037	0.019	0.581
Rmeas	0.040	0.021	0.634
Rpim	0.016	0.008	0.250
Number of reflections	51069	2770	2513
<I/σ(I)>	8
Completeness [%]	99.5	99.2	99.5
Redundancy	6.3	6.1	6.1
CC(1/2)		0.999	0.885

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	298	20% (w/v) PEGMME 2000, 100 mM Tris pH 8.5, and 200 mM Tris-methylamine N-oxide dehydrate