6XJ1

Crystal Structure of CDC15 F-BAR Domain from Schizosaccharomyces pombe

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-01
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	0.97872
Spacegroup name	P 1 21 1
Unit cell lengths	54.777, 75.171, 93.239
Unit cell angles	90.00, 95.33, 90.00

Refinement procedure

Resolution	37.590 - 3.520
R-factor	0.2691
Rwork	0.267
R-free	0.30960
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5c1f
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.590	2.430
High resolution limit [Å]	2.350	2.350
Rmerge	0.099	0.354
Number of reflections	59833	688
<I/σ(I)>	11.8	2.6
Completeness [%]	98.5	88.2
Redundancy	3.3	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7	293	100 mM HEPES (pH 7.0), 1.1 M Sodium Malonate Dibasic Monohydrate (pH 7.0) and 1% Jeffamine ED 2003 (pH 7.0)