6XI6
Hierarchical design of multi-scale protein complexes by combinatorial assembly of oligomeric helical bundle and repeat protein building blocks
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2019-09-12 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97918 |
Spacegroup name | H 3 |
Unit cell lengths | 101.970, 101.970, 78.444 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 38.480 - 2.690 |
R-factor | 0.2268 |
Rwork | 0.221 |
R-free | 0.27720 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Designed model |
RMSD bond length | 0.001 |
RMSD bond angle | 0.306 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.17rc1_3605) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.480 | 2.780 |
High resolution limit [Å] | 2.690 | 2.690 |
Number of reflections | 8434 | 844 |
<I/σ(I)> | 11.4 | 2.3 |
Completeness [%] | 99.6 | 99.41 |
Redundancy | 4.8 | 2.3 |
CC(1/2) | 0.997 | 0.889 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M MgCl2, 0.1 TrisCl pH 8.5. 10 % Glycerol and 25 (v/v) 1,2-Propanediol |