6XI1
Crystal structure of tetra-tandem repeat in extending RTX adhesin from Aeromonas hydrophila
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-02-12 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.97949 |
| Spacegroup name | P 1 |
| Unit cell lengths | 47.670, 50.370, 124.600 |
| Unit cell angles | 97.56, 98.24, 101.72 |
Refinement procedure
| Resolution | 48.638 - 1.750 |
| Rwork | 0.181 |
| R-free | 0.20980 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.574 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | AutoSol |
| Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.640 | 1.780 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.081 | 0.830 |
| Rmeas | 0.094 | 0.960 |
| Rpim | 0.048 | 0.483 |
| Number of reflections | 108730 | 5282 |
| <I/σ(I)> | 10 | 1.9 |
| Completeness [%] | 97.3 | 95.3 |
| Redundancy | 3.9 | 4 |
| CC(1/2) | 0.998 | 0.859 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 4.6 | 294 | PEG 20,000, sodium acetate |
