6XGY

Crystal structure of E. coli MlaFB ABC transport subunits in the dimeric state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-08-12
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.11583
Spacegroup name	P 32 2 1
Unit cell lengths	102.710, 102.710, 89.710
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.570 - 2.900
R-factor	0.1979
Rwork	0.196
R-free	0.24290
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ouk 3f43
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.570	3.004
High resolution limit [Å]	2.900	2.900
Number of reflections	12466	1213
<I/σ(I)>	16.09
Completeness [%]	93.1
Redundancy	19.7
CC(1/2)	0.999	0.349

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277.15	0.2 M magnesium formate