6XGW
ISCth4 transposase, pre-reaction complex, PRC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 95 |
| Detector technology | PIXEL |
| Collection date | 2018-11-29 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 89.578, 99.050, 156.088 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.480 - 3.500 |
| R-factor | 0.2515 |
| Rwork | 0.249 |
| R-free | 0.29640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6xg8 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.571 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.070 | 3.590 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmerge | 0.079 | 0.844 |
| Rmeas | 0.097 | |
| Number of reflections | 17976 | 1323 |
| <I/σ(I)> | 10.94 | 1.78 |
| Completeness [%] | 99.2 | 99.66 |
| Redundancy | 3.6 | 3.87 |
| CC(1/2) | 0.799 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293.15 | sodium cacodylate, calcium chloride, ammonium acetate, PEG4000 |
