6XGW

ISCth4 transposase, pre-reaction complex, PRC

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	95
Detector technology	PIXEL
Collection date	2018-11-29
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	89.578, 99.050, 156.088
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.480 - 3.500
R-factor	0.2515
Rwork	0.249
R-free	0.29640
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6xg8
RMSD bond length	0.003
RMSD bond angle	0.571
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	BUSTER

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.070	3.590
High resolution limit [Å]	3.500	3.500
Rmerge	0.079	0.844
Rmeas	0.097
Number of reflections	17976	1323
<I/σ(I)>	10.94	1.78
Completeness [%]	99.2	99.66
Redundancy	3.6	3.87
CC(1/2)		0.799

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293.15	sodium cacodylate, calcium chloride, ammonium acetate, PEG4000