6XGU
Crystal Structure of KRAS-Q61R (GMPPNP-bound) in complex with RAS-binding domain (RBD) and cysteine-rich domain (CRD) of RAF1/CRAF
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-12-09 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9791 |
Spacegroup name | P 6 2 2 |
Unit cell lengths | 132.320, 132.320, 89.910 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 48.320 - 2.700 |
R-factor | 0.1922 |
Rwork | 0.187 |
R-free | 0.23570 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6xhb |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 48.320 | 48.320 | 2.950 |
High resolution limit [Å] | 2.700 | 8.220 | 2.780 |
Rmerge | 0.069 | 0.034 | 0.834 |
Rmeas | 0.073 | 0.036 | 0.870 |
Number of reflections | 13291 | 547 | 1912 |
<I/σ(I)> | 25.35 | 63.4 | 2.96 |
Completeness [%] | 99.8 | 98.4 | 99.5 |
Redundancy | 12.125 | 10.223 | 12.105 |
CC(1/2) | 0.998 | 0.998 | 0.927 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.8 | 293 | 100 mM Tris 7.8, 200 mM KBr, 200 mM KSCN, 3% PGA, 0.35 mM D-myo-phosphatidylinositol 3,4,5-triphosphate |